NAME
perlprimer - graphically specify amplicon of DNA or mRNA sequences and
design primers
SYNOPSIS
perlprimer
DESCRIPTION
PerlPrimer calculates primer melting temperature using J. SantaLucia’s
extensive nearest-neighbour thermodynamic parameters. To adjust for the
salt conditions of the PCR, PerlPrimer uses the empirical formula
derived by von Ahsen, et al. (2001) and allows the user to specify the
concentration of Mg2+, dNTPs and primers, or use standard PCR
conditions. The result is a highly accurate prediction of primer
melting temperature, giving rise to a maximum yeild of product when
amplified. PerlPrimer is written in Perl and requires Perl/Tk. In
addition, for QPCR functionality PerlPrimer requires the open-source
Spidey executable from NCBI. The program is designed to be cross-
platform compatible and has been developed and tested on both Microsoft
Windows and GNU/Linux-based operating systems. Users have also reported
success using the program under Mac OS X.
SEE ALSO
A very nice tutorial on http://perlprimer.sourceforge.net is also
distributed as a debian package perlprimer-doc.
AUTHOR
perlprimer was written by Owen Marshall <owenjm@users.sourceforge.net>
This manual page was assembled by Steffen Moeller
<steffen_moeller@gmx.de>, for the Debian project (but may be used by
others).
6 January 2005